Measurement

Part:BBa_K2066028:Experience

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-06)


William and Mary iGEM 2016 sequence confirmed this part.

We also validated its fluorescence, induction capability, and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry.

Validation of fluorescence and induction capability: William_and_Mary_28_IC3_Induction.png

We did not obtain plots for repressor functionality due to a data storage error, but we did confirm that with fixed Arabinose concentration at 1 mM and variable IPTG induction over 0 - 10 mM range, that FL3 fluorescence levels (to detect mCherry) were were constant across the induction (in arbitrary fluorescence units) while the FL1 fluorescence levels (to detect sfGFP from BBa_K2066014) increased with induction (in arbitrary fluorescence units).

Applications of BBa_K2066028

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